a31 mcherry arp3 gift (Addgene inc)
Addgene inc is a verified supplier 
92
Structured Review
Addgene inc
a31 mcherry arp3 gift
A31 Mcherry Arp3 Gift, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a31+mcherry+arp3+gift/mCherry-ARP3-C-12+(Plasmid+%2354981)/pm38968072-795-65-71
Average 92 stars, based on 1 article reviews
A31 Mcherry Arp3 Gift, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a31+mcherry+arp3+gift/mCherry-ARP3-C-12+(Plasmid+%2354981)/pm38968072-795-65-71
Average 92 stars, based on 1 article reviews
a31 mcherry arp3 gift - by Bioz Stars,
2026-09
92/100 stars
Images
Related Articles
Recombinant:Article Title: Competition and synergy of Arp2/3 and formins in nucleating actin waves. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CK-666 Sigma-Aldrich SML0006 Cytochalasin-D Sigma-Aldrich C8273 FBS Millipore Sigma F4135 MEM Life Technologies 11095098 TrypLE Express Invitrogen 12604021 Puromycin Gibco A1113803 Experimental models: Cell lines RBL-2H3 cell ATCC Cat#: CRL-2256; RRID:CVCL_0591 RBL-2H3 cell stably expressing Cdc42 BD-GFP – Wu Lab Cell Line ID: B-ae Recombinant DNA GFP-Arp3 Gift from Dorothy Schafer Wu Lab Plasmid ID: A31 mCherry-Arp3 Gift from Michael Davidson (Addgene #54981) Wu Lab Plasmid ID: A36 LifeAct-GFP Gift from Roland Wedlich-Solber Wu Lab Plasmid ID: A11 LifeAct-mRuby Subcloned by replacing GFP of LifeAct-GFP with mRuby tag Wu Lab Plasmid ID: A12 FMNL1-GFP Gift from Michael Rosen Wu Lab Plasmid ID: A98 GFP-CA-FMNL1 Gift from Michael Rosen Wu Lab Plasmid ID: A98i GFP-FMNL1CT Gift from Michael Rosen Wu Lab Plasmid ID: A98c GFP-mini-FMNL1-T126D (inactive) Gift from Michael Rosen Wu Lab Plasmid ID: A98g GFP-mini-FMNL1-V281E (active) Gift from Michael Rosen Wu Lab Plasmid ID: A98h GFP-mDia3 Gift from Shuh Narumiya Wu Lab Plasmid ID: A99 mCherry-mDia3 Subcloned from GFP-mDia3, gift from Shuh Narumiya Wu Lab Plasmid ID: A99c mCherry-CA-mDia3 Subcloned from mCherry-mDia3 with overlap extension PCR Wu Lab Plasmid ID: A99b PM-mScarlet Subcloned from PM-miRFP670 with PCR to replace fluorescent tag Wu Lab Plasmid ID: M02b Cdc42 BD-GFP Subcloned into pEGFP-N1 vector from pET23-CBD-(-PP)-EGFP Wu Lab Plasmid ID: G011 FBP17-EGFP Gift from Pietro De Camilli Wu Lab Plasmid ID: F011 GFP-SHIP1 Gift from Pietro De Camilli Wu Lab Plasmid ID: P87 GFP-N-WASP Subcloned into EGFP-C1 vector Wu Lab Plasmid ID: A41 Software and algorithms Custom codes for analysis Written in-house https://github.com/min-wu-lab/2024- Chua-et-al, https://doi.org/10.5281/ zenodo.8083400 Fiji (ImageJ) NIH http://fiji.sc/ Prism 10 Graphpad N/A MATLAB Mathworks N/A Other Neon Transfection System 10 mL Kit Life Technologies MPK1096 35 mm Dish | No. 1.5 Coverslip | 20 mm Glass Diameter | Uncoated Mattek Corporation P35G-1.5-20-C 18 Cell Reports 43, 114423, July 23, 2024 Stable Transfection:Article Title: Competition and synergy of Arp2/3 and formins in nucleating actin waves. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CK-666 Sigma-Aldrich SML0006 Cytochalasin-D Sigma-Aldrich C8273 FBS Millipore Sigma F4135 MEM Life Technologies 11095098 TrypLE Express Invitrogen 12604021 Puromycin Gibco A1113803 Experimental models: Cell lines RBL-2H3 cell ATCC Cat#: CRL-2256; RRID:CVCL_0591 RBL-2H3 cell stably expressing Cdc42 BD-GFP – Wu Lab Cell Line ID: B-ae Recombinant DNA GFP-Arp3 Gift from Dorothy Schafer Wu Lab Plasmid ID: A31 mCherry-Arp3 Gift from Michael Davidson (Addgene #54981) Wu Lab Plasmid ID: A36 LifeAct-GFP Gift from Roland Wedlich-Solber Wu Lab Plasmid ID: A11 LifeAct-mRuby Subcloned by replacing GFP of LifeAct-GFP with mRuby tag Wu Lab Plasmid ID: A12 FMNL1-GFP Gift from Michael Rosen Wu Lab Plasmid ID: A98 GFP-CA-FMNL1 Gift from Michael Rosen Wu Lab Plasmid ID: A98i GFP-FMNL1CT Gift from Michael Rosen Wu Lab Plasmid ID: A98c GFP-mini-FMNL1-T126D (inactive) Gift from Michael Rosen Wu Lab Plasmid ID: A98g GFP-mini-FMNL1-V281E (active) Gift from Michael Rosen Wu Lab Plasmid ID: A98h GFP-mDia3 Gift from Shuh Narumiya Wu Lab Plasmid ID: A99 mCherry-mDia3 Subcloned from GFP-mDia3, gift from Shuh Narumiya Wu Lab Plasmid ID: A99c mCherry-CA-mDia3 Subcloned from mCherry-mDia3 with overlap extension PCR Wu Lab Plasmid ID: A99b PM-mScarlet Subcloned from PM-miRFP670 with PCR to replace fluorescent tag Wu Lab Plasmid ID: M02b Cdc42 BD-GFP Subcloned into pEGFP-N1 vector from pET23-CBD-(-PP)-EGFP Wu Lab Plasmid ID: G011 FBP17-EGFP Gift from Pietro De Camilli Wu Lab Plasmid ID: F011 GFP-SHIP1 Gift from Pietro De Camilli Wu Lab Plasmid ID: P87 GFP-N-WASP Subcloned into EGFP-C1 vector Wu Lab Plasmid ID: A41 Software and algorithms Custom codes for analysis Written in-house https://github.com/min-wu-lab/2024- Chua-et-al, https://doi.org/10.5281/ zenodo.8083400 Fiji (ImageJ) NIH http://fiji.sc/ Prism 10 Graphpad N/A MATLAB Mathworks N/A Other Neon Transfection System 10 mL Kit Life Technologies MPK1096 35 mm Dish | No. 1.5 Coverslip | 20 mm Glass Diameter | Uncoated Mattek Corporation P35G-1.5-20-C 18 Cell Reports 43, 114423, July 23, 2024 Expressing:Article Title: Competition and synergy of Arp2/3 and formins in nucleating actin waves. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CK-666 Sigma-Aldrich SML0006 Cytochalasin-D Sigma-Aldrich C8273 FBS Millipore Sigma F4135 MEM Life Technologies 11095098 TrypLE Express Invitrogen 12604021 Puromycin Gibco A1113803 Experimental models: Cell lines RBL-2H3 cell ATCC Cat#: CRL-2256; RRID:CVCL_0591 RBL-2H3 cell stably expressing Cdc42 BD-GFP – Wu Lab Cell Line ID: B-ae Recombinant DNA GFP-Arp3 Gift from Dorothy Schafer Wu Lab Plasmid ID: A31 mCherry-Arp3 Gift from Michael Davidson (Addgene #54981) Wu Lab Plasmid ID: A36 LifeAct-GFP Gift from Roland Wedlich-Solber Wu Lab Plasmid ID: A11 LifeAct-mRuby Subcloned by replacing GFP of LifeAct-GFP with mRuby tag Wu Lab Plasmid ID: A12 FMNL1-GFP Gift from Michael Rosen Wu Lab Plasmid ID: A98 GFP-CA-FMNL1 Gift from Michael Rosen Wu Lab Plasmid ID: A98i GFP-FMNL1CT Gift from Michael Rosen Wu Lab Plasmid ID: A98c GFP-mini-FMNL1-T126D (inactive) Gift from Michael Rosen Wu Lab Plasmid ID: A98g GFP-mini-FMNL1-V281E (active) Gift from Michael Rosen Wu Lab Plasmid ID: A98h GFP-mDia3 Gift from Shuh Narumiya Wu Lab Plasmid ID: A99 mCherry-mDia3 Subcloned from GFP-mDia3, gift from Shuh Narumiya Wu Lab Plasmid ID: A99c mCherry-CA-mDia3 Subcloned from mCherry-mDia3 with overlap extension PCR Wu Lab Plasmid ID: A99b PM-mScarlet Subcloned from PM-miRFP670 with PCR to replace fluorescent tag Wu Lab Plasmid ID: M02b Cdc42 BD-GFP Subcloned into pEGFP-N1 vector from pET23-CBD-(-PP)-EGFP Wu Lab Plasmid ID: G011 FBP17-EGFP Gift from Pietro De Camilli Wu Lab Plasmid ID: F011 GFP-SHIP1 Gift from Pietro De Camilli Wu Lab Plasmid ID: P87 GFP-N-WASP Subcloned into EGFP-C1 vector Wu Lab Plasmid ID: A41 Software and algorithms Custom codes for analysis Written in-house https://github.com/min-wu-lab/2024- Chua-et-al, https://doi.org/10.5281/ zenodo.8083400 Fiji (ImageJ) NIH http://fiji.sc/ Prism 10 Graphpad N/A MATLAB Mathworks N/A Other Neon Transfection System 10 mL Kit Life Technologies MPK1096 35 mm Dish | No. 1.5 Coverslip | 20 mm Glass Diameter | Uncoated Mattek Corporation P35G-1.5-20-C 18 Cell Reports 43, 114423, July 23, 2024 Plasmid Preparation:Article Title: Competition and synergy of Arp2/3 and formins in nucleating actin waves. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CK-666 Sigma-Aldrich SML0006 Cytochalasin-D Sigma-Aldrich C8273 FBS Millipore Sigma F4135 MEM Life Technologies 11095098 TrypLE Express Invitrogen 12604021 Puromycin Gibco A1113803 Experimental models: Cell lines RBL-2H3 cell ATCC Cat#: CRL-2256; RRID:CVCL_0591 RBL-2H3 cell stably expressing Cdc42 BD-GFP – Wu Lab Cell Line ID: B-ae Recombinant DNA GFP-Arp3 Gift from Dorothy Schafer Wu Lab Plasmid ID: A31 mCherry-Arp3 Gift from Michael Davidson (Addgene #54981) Wu Lab Plasmid ID: A36 LifeAct-GFP Gift from Roland Wedlich-Solber Wu Lab Plasmid ID: A11 LifeAct-mRuby Subcloned by replacing GFP of LifeAct-GFP with mRuby tag Wu Lab Plasmid ID: A12 FMNL1-GFP Gift from Michael Rosen Wu Lab Plasmid ID: A98 GFP-CA-FMNL1 Gift from Michael Rosen Wu Lab Plasmid ID: A98i GFP-FMNL1CT Gift from Michael Rosen Wu Lab Plasmid ID: A98c GFP-mini-FMNL1-T126D (inactive) Gift from Michael Rosen Wu Lab Plasmid ID: A98g GFP-mini-FMNL1-V281E (active) Gift from Michael Rosen Wu Lab Plasmid ID: A98h GFP-mDia3 Gift from Shuh Narumiya Wu Lab Plasmid ID: A99 mCherry-mDia3 Subcloned from GFP-mDia3, gift from Shuh Narumiya Wu Lab Plasmid ID: A99c mCherry-CA-mDia3 Subcloned from mCherry-mDia3 with overlap extension PCR Wu Lab Plasmid ID: A99b PM-mScarlet Subcloned from PM-miRFP670 with PCR to replace fluorescent tag Wu Lab Plasmid ID: M02b Cdc42 BD-GFP Subcloned into pEGFP-N1 vector from pET23-CBD-(-PP)-EGFP Wu Lab Plasmid ID: G011 FBP17-EGFP Gift from Pietro De Camilli Wu Lab Plasmid ID: F011 GFP-SHIP1 Gift from Pietro De Camilli Wu Lab Plasmid ID: P87 GFP-N-WASP Subcloned into EGFP-C1 vector Wu Lab Plasmid ID: A41 Software and algorithms Custom codes for analysis Written in-house https://github.com/min-wu-lab/2024- Chua-et-al, https://doi.org/10.5281/ zenodo.8083400 Fiji (ImageJ) NIH http://fiji.sc/ Prism 10 Graphpad N/A MATLAB Mathworks N/A Other Neon Transfection System 10 mL Kit Life Technologies MPK1096 35 mm Dish | No. 1.5 Coverslip | 20 mm Glass Diameter | Uncoated Mattek Corporation P35G-1.5-20-C 18 Cell Reports 43, 114423, July 23, 2024 Polymerase Chain Reaction:Article Title: Competition and synergy of Arp2/3 and formins in nucleating actin waves. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CK-666 Sigma-Aldrich SML0006 Cytochalasin-D Sigma-Aldrich C8273 FBS Millipore Sigma F4135 MEM Life Technologies 11095098 TrypLE Express Invitrogen 12604021 Puromycin Gibco A1113803 Experimental models: Cell lines RBL-2H3 cell ATCC Cat#: CRL-2256; RRID:CVCL_0591 RBL-2H3 cell stably expressing Cdc42 BD-GFP – Wu Lab Cell Line ID: B-ae Recombinant DNA GFP-Arp3 Gift from Dorothy Schafer Wu Lab Plasmid ID: A31 mCherry-Arp3 Gift from Michael Davidson (Addgene #54981) Wu Lab Plasmid ID: A36 LifeAct-GFP Gift from Roland Wedlich-Solber Wu Lab Plasmid ID: A11 LifeAct-mRuby Subcloned by replacing GFP of LifeAct-GFP with mRuby tag Wu Lab Plasmid ID: A12 FMNL1-GFP Gift from Michael Rosen Wu Lab Plasmid ID: A98 GFP-CA-FMNL1 Gift from Michael Rosen Wu Lab Plasmid ID: A98i GFP-FMNL1CT Gift from Michael Rosen Wu Lab Plasmid ID: A98c GFP-mini-FMNL1-T126D (inactive) Gift from Michael Rosen Wu Lab Plasmid ID: A98g GFP-mini-FMNL1-V281E (active) Gift from Michael Rosen Wu Lab Plasmid ID: A98h GFP-mDia3 Gift from Shuh Narumiya Wu Lab Plasmid ID: A99 mCherry-mDia3 Subcloned from GFP-mDia3, gift from Shuh Narumiya Wu Lab Plasmid ID: A99c mCherry-CA-mDia3 Subcloned from mCherry-mDia3 with overlap extension PCR Wu Lab Plasmid ID: A99b PM-mScarlet Subcloned from PM-miRFP670 with PCR to replace fluorescent tag Wu Lab Plasmid ID: M02b Cdc42 BD-GFP Subcloned into pEGFP-N1 vector from pET23-CBD-(-PP)-EGFP Wu Lab Plasmid ID: G011 FBP17-EGFP Gift from Pietro De Camilli Wu Lab Plasmid ID: F011 GFP-SHIP1 Gift from Pietro De Camilli Wu Lab Plasmid ID: P87 GFP-N-WASP Subcloned into EGFP-C1 vector Wu Lab Plasmid ID: A41 Software and algorithms Custom codes for analysis Written in-house https://github.com/min-wu-lab/2024- Chua-et-al, https://doi.org/10.5281/ zenodo.8083400 Fiji (ImageJ) NIH http://fiji.sc/ Prism 10 Graphpad N/A MATLAB Mathworks N/A Other Neon Transfection System 10 mL Kit Life Technologies MPK1096 35 mm Dish | No. 1.5 Coverslip | 20 mm Glass Diameter | Uncoated Mattek Corporation P35G-1.5-20-C 18 Cell Reports 43, 114423, July 23, 2024 Software:Article Title: Competition and synergy of Arp2/3 and formins in nucleating actin waves. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CK-666 Sigma-Aldrich SML0006 Cytochalasin-D Sigma-Aldrich C8273 FBS Millipore Sigma F4135 MEM Life Technologies 11095098 TrypLE Express Invitrogen 12604021 Puromycin Gibco A1113803 Experimental models: Cell lines RBL-2H3 cell ATCC Cat#: CRL-2256; RRID:CVCL_0591 RBL-2H3 cell stably expressing Cdc42 BD-GFP – Wu Lab Cell Line ID: B-ae Recombinant DNA GFP-Arp3 Gift from Dorothy Schafer Wu Lab Plasmid ID: A31 mCherry-Arp3 Gift from Michael Davidson (Addgene #54981) Wu Lab Plasmid ID: A36 LifeAct-GFP Gift from Roland Wedlich-Solber Wu Lab Plasmid ID: A11 LifeAct-mRuby Subcloned by replacing GFP of LifeAct-GFP with mRuby tag Wu Lab Plasmid ID: A12 FMNL1-GFP Gift from Michael Rosen Wu Lab Plasmid ID: A98 GFP-CA-FMNL1 Gift from Michael Rosen Wu Lab Plasmid ID: A98i GFP-FMNL1CT Gift from Michael Rosen Wu Lab Plasmid ID: A98c GFP-mini-FMNL1-T126D (inactive) Gift from Michael Rosen Wu Lab Plasmid ID: A98g GFP-mini-FMNL1-V281E (active) Gift from Michael Rosen Wu Lab Plasmid ID: A98h GFP-mDia3 Gift from Shuh Narumiya Wu Lab Plasmid ID: A99 mCherry-mDia3 Subcloned from GFP-mDia3, gift from Shuh Narumiya Wu Lab Plasmid ID: A99c mCherry-CA-mDia3 Subcloned from mCherry-mDia3 with overlap extension PCR Wu Lab Plasmid ID: A99b PM-mScarlet Subcloned from PM-miRFP670 with PCR to replace fluorescent tag Wu Lab Plasmid ID: M02b Cdc42 BD-GFP Subcloned into pEGFP-N1 vector from pET23-CBD-(-PP)-EGFP Wu Lab Plasmid ID: G011 FBP17-EGFP Gift from Pietro De Camilli Wu Lab Plasmid ID: F011 GFP-SHIP1 Gift from Pietro De Camilli Wu Lab Plasmid ID: P87 GFP-N-WASP Subcloned into EGFP-C1 vector Wu Lab Plasmid ID: A41 Software and algorithms Custom codes for analysis Written in-house https://github.com/min-wu-lab/2024- Chua-et-al, https://doi.org/10.5281/ zenodo.8083400 Fiji (ImageJ) NIH http://fiji.sc/ Prism 10 Graphpad N/A MATLAB Mathworks N/A Other Neon Transfection System 10 mL Kit Life Technologies MPK1096 35 mm Dish | No. 1.5 Coverslip | 20 mm Glass Diameter | Uncoated Mattek Corporation P35G-1.5-20-C 18 Cell Reports 43, 114423, July 23, 2024 Transfection:Article Title: Competition and synergy of Arp2/3 and formins in nucleating actin waves. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CK-666 Sigma-Aldrich SML0006 Cytochalasin-D Sigma-Aldrich C8273 FBS Millipore Sigma F4135 MEM Life Technologies 11095098 TrypLE Express Invitrogen 12604021 Puromycin Gibco A1113803 Experimental models: Cell lines RBL-2H3 cell ATCC Cat#: CRL-2256; RRID:CVCL_0591 RBL-2H3 cell stably expressing Cdc42 BD-GFP – Wu Lab Cell Line ID: B-ae Recombinant DNA GFP-Arp3 Gift from Dorothy Schafer Wu Lab Plasmid ID: A31 mCherry-Arp3 Gift from Michael Davidson (Addgene #54981) Wu Lab Plasmid ID: A36 LifeAct-GFP Gift from Roland Wedlich-Solber Wu Lab Plasmid ID: A11 LifeAct-mRuby Subcloned by replacing GFP of LifeAct-GFP with mRuby tag Wu Lab Plasmid ID: A12 FMNL1-GFP Gift from Michael Rosen Wu Lab Plasmid ID: A98 GFP-CA-FMNL1 Gift from Michael Rosen Wu Lab Plasmid ID: A98i GFP-FMNL1CT Gift from Michael Rosen Wu Lab Plasmid ID: A98c GFP-mini-FMNL1-T126D (inactive) Gift from Michael Rosen Wu Lab Plasmid ID: A98g GFP-mini-FMNL1-V281E (active) Gift from Michael Rosen Wu Lab Plasmid ID: A98h GFP-mDia3 Gift from Shuh Narumiya Wu Lab Plasmid ID: A99 mCherry-mDia3 Subcloned from GFP-mDia3, gift from Shuh Narumiya Wu Lab Plasmid ID: A99c mCherry-CA-mDia3 Subcloned from mCherry-mDia3 with overlap extension PCR Wu Lab Plasmid ID: A99b PM-mScarlet Subcloned from PM-miRFP670 with PCR to replace fluorescent tag Wu Lab Plasmid ID: M02b Cdc42 BD-GFP Subcloned into pEGFP-N1 vector from pET23-CBD-(-PP)-EGFP Wu Lab Plasmid ID: G011 FBP17-EGFP Gift from Pietro De Camilli Wu Lab Plasmid ID: F011 GFP-SHIP1 Gift from Pietro De Camilli Wu Lab Plasmid ID: P87 GFP-N-WASP Subcloned into EGFP-C1 vector Wu Lab Plasmid ID: A41 Software and algorithms Custom codes for analysis Written in-house https://github.com/min-wu-lab/2024- Chua-et-al, https://doi.org/10.5281/ zenodo.8083400 Fiji (ImageJ) NIH http://fiji.sc/ Prism 10 Graphpad N/A MATLAB Mathworks N/A Other Neon Transfection System 10 mL Kit Life Technologies MPK1096 35 mm Dish | No. 1.5 Coverslip | 20 mm Glass Diameter | Uncoated Mattek Corporation P35G-1.5-20-C 18 Cell Reports 43, 114423, July 23, 2024 |